Volume 3, Issue 1 p. 96-100
Article

Rapid determination of double-bond positions in monoenoic fatty acids by periodate-permanganate oxidation

Donald T. Downing

Donald T. Downing

Departments of Dermatology and Biochemistry, Boston University School of Medicine, Boston, Massachusetts

Department of Dermatology, Evans Memorial Department of Clinical Research, University Hospital, Boston University Medical Center, Boston, Massachusetts

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Richard S. Greene

Richard S. Greene

Departments of Dermatology and Biochemistry, Boston University School of Medicine, Boston, Massachusetts

Department of Dermatology, Evans Memorial Department of Clinical Research, University Hospital, Boston University Medical Center, Boston, Massachusetts

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First published: 01 January 1968
Citations: 64

Abstract

Tetramethylammonium hydroxide has been used in the extraction and pyrolysis methylation of the carboxylic acids produced by periodate-permanganate oxidation of monounsaturated fatty acid methyl esters. This modification of the von Rudloff procedure allows rapid determination of double-bond positions and analysis of mixtures of positional isomers of monoenoic fatty acids.